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PTTG1 might be a candidate downstream gene of PRR11 and involved in the cell cycle. (B) Heat map of the cell cycle–related genes from both TCGA and RNA-seq. The liver, lung, and pancreatic cancer cell lines (HepG2, NCI-H460, and BxPC3) were gifts from Dr. Peng Wang, Sun Yat-sen University Cancer Hospital, Guangzhou, China.
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PRR11 overexpression confers resistance to antiestrogens
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Look for a mentor in your chosen career who can help you build a long-term strategy for making and keeping money. MCF7 LTED (5 × 104/well) cells were seeded in 8-well chamber slides (Lab-Tek, ) in triplicate. HEK293 cells (5 × 104) were seeded 24 h post-transfection with pLX302-PRR11-V5, pLenti7.3-PIK3R1-Flag, and pLenti7.3-PIK3R1-HA. P85 (Cat# , Millipore), V5 (Cat# 13202, Cell Signaling Technology), HA (Cat# 3724, Cell Signaling Technology) and Flag (Cat# 8146, Cell Signaling Technology) antibodies were used for this assay.
One such example is 12-year-old Ryan, who has made quite a fortune by reviewing new toys and playing with them. Many of us dream of becoming incredibly wealthy while still young, don’t we? It’s often said that money can’t buy happiness, but it can certainly make life more enjoyable and convenient. The new markdown and rich text formatting features in Windows 11 Notepad make it a perfect tool for everyone to draft notes. Simply update to the latest version via the Microsoft Store and give it a spin! If you’ve got any questions or suggestions, leave them in the comments.
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You can earn spin tickets through deposits, daily logins, or simply by registering. Each spin requires 5 tickets, and winnings come with a 5× wagering requirement. By making a bank transfer deposit of more than 1,000 INR, you get a 6% bonus. This is a great promotion if you are using a reliable and familiar payment method for larger deposits.
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MCF10A pLX302-PRR11 or -LacZ cells were grown ± 1 µM taselisib or ± 1 µM alpelisib for 10 days. Cells were fixed with cold-iced methanol for 10 min at −20 °C and then stained with 0.5% crystal violet solution for 10 min at room temperature. Stained monolayers were imaged using Gelcount mammalian cell colony counter (Oxford Optronix). For quantification, cell monolayers were dissolved with 10% acetic acid for 15 min at room temperature. Supernatants were transferred into 96-well plates for measuring absorbance at 560 nm using GloMax Discover microplate reader (Promega).
Use Rich to make your command line applications visually appealing and present data in a more readable way. Rich can also be a useful debugging aid by pretty printing and syntax highlighting data structures. Rich is a Python library for writing rich text (with color and style) to the terminal, and for displaying advanced content such as tables, markdown, and syntax highlighted code. After writing tech-related things and other long-form content for 2-3 years, I found my calling with games and anime.
H, i MCF7 LTED cells transfected with control or PRR11 siRNA (h) and MDA-MB-175VII cells transduced with pLX302-LacZ or pLX302-PRR11 (i) were serum-starved for 24 h and then treated with 100 nM insulin for 10 min. Cell lysates were prepared and immunoprecipitated with a p110α antibody rich11 site or IgG. J MDA-MB-175VII cells transduced with pLX302-LacZ or pLX302-PRR11 were serum-starved for 24 h and then treated with 100 nM insulin for 10 min.
